Point-of-Care Testing – Biosensor for Norepinephrine Determination
Abstract
An useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)-9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with thin polymeric film. This sensing arrangement utilized the catalytic oxidation of NE to norepinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of enzyme – laccase. With the optimized conditions, the analytical performance demonstrated selectivity in a wide linear range (0.1–200x10-6 M) with a detection limit of 240 nM and a quantification limit of 365 nM. Moreover, the method was successfully applied for selective NE determination in the presence of interfering substances.
References
H. Gunduz-Bruce, “The acute effects of NMDA antagonism: from the rodent to the human brain”, Brain Research Reviews, vol. 60, May 2009, pp. 279–286
S. J. G. Lewis, R. A. Barker, “Understanding the dopaminergic deficits in Parkinson’s disease: Insightsinto disease heterogeneity”, Journal of Clinical Neuroscience, vol. 16, Aug. 2009, pp. 620–625
E. R. Peskind, R. Elrod, D. J. Dobie, M. Pascualy, E. Petrie, C. Jensen, K. Brodkin, S. Murra, R. C. Veith, M. A. Raskind, “Cerebrospinal Fluid Epinephrine in Alzheimer's Disease and Normal Aging”, Neuropsychopharmacology, vol. 19, Dec. 1998, pp. 465–471
E. Wierzbicka, M. Szultka-Młynska, B. Buszewski, G. D. Sulka, “Epinephrine sensing at nanostructured Au electrode and determination its oxidative metabolism”, Sensors and Actuators B, vol. 237, Dec. 2016, pp. 206–215
P. Nagaraja, R.A. Vasantha, K.R. Sunitha, “A sensitive and selective spectrophotometric estimation of catechol derivatives in pharmaceutical preparations”, Talanta, vol. 55, Dec. 2001, pp. 1039-1046
V. Carrera, E. Sabater, E. Vilanova, M.A. Sogorb, “A simple and rapid HPLC-MS method for the simultaneous determination of epinephrine, norepinephrine, dopamine and 5-hydroxytryptamine: application to the secretion of bovine chromaffin cell cultures”, Journal of Chromatography B, vol. 847, Mar. 2007, pp. 88-94
K.E. Secor, T.E. Glass, “Selective amine recognition: development of a chemosensor for dopamine and norepinephrine”, Organic Letters, vol. 6, Oct. 2004, pp. 3727-3730
N. W. Barnett, B.J. Hindson, S.W. Lewis, “Determination of 5-hydroxytryptamine (serotonin) and related indoles by flow injection analysis with acidic potassium permanganate chemiluminescence detection”, Analytica Chimica Acta, vol. 362, May 1998, pp. 131–139
F. Artigas, M.J. Sarrias, E. Martinez, E. Gelpi, “Serotonin in body fluids: characterization of human plasmatic and cerebrospinal fluid pools by means of a new HPLC method”, Life Science, vol. 37, Aug. 1985, pp. 441-447
L.A. Kartsova, A.A. Sidorova, V.A. Kazakov, E.A. Bessonova, A.Y. Yashin, “Determination of Catecholamines by Capillary Electrophoresis and Reversed-Phase High-Performance Liquid Chromatography”, Journal of Analytical Chemistry, vol. 59, Aug. 2004, pp. 737-741
K.J. Samdani, J.S. Samdani, N.H. Kim, J.H. Lee, “FeMoO4 based, enzyme-free electrochemical biosensor for ultrasensitive detection of norepinephrine” Biosensors and Bioelectronics, vol. 81, Jul. 2016, pp. 445-453
Y. Wang, S. Wang, L. Tao, Q. Min, J. Hiang, Q. Wang, J. Hie, Y. Yue, S. Wu, X. Li, H. Ding, “A disposable electrochemical sensor for simultaneous determination of norepinephrine and serotonin in rat cerebrospinal fluid based on MWNTs-ZnO/chitosan composites modified screen-printed electrode”, Biosensors and Bioelectronics, vol. 65, Mar. 2015, pp. 31-38
L-P. Lu, S-Q. Wang, X-Q. Lin, “Fabrication of layer-by-layer deposited multilayer films containing DNA and gold nanoparticle for norepinephrine biosensor”, Analytica Chimica Acta, vol. 519, Aug. 2004, pp. 161-166
D.K. Drain, E.P. Hyle, F. Naubary, K.A. Freedberg, D. Wilson, W.R. Bishai, “Evaluating Diagnostic Point-of-Care Tests in Resource-Limited Settings”, Lancet Infectious Diseases, vol. 14, Mar. 2014, pp. 239–249
R.W. Peeling, K.K. Holmes, D. Mabey, “Rapid tests for sexually transmitted infections (STIs): the way forward”, Sexually Transmmited Infection, vol. 82, Dec. 2006, pp. v1–v6
A. St John, C.P. Price, “Existing and emerging technologies for point-of-care testing”, The Clinical Biochemist Reviews, vol. 35, Aug. 2014, pp. 155–167
J.W. Schultze, H. Karabulut, “Application potential of conducting polymers”, Electrochimica Acta, vol. 50, Feb. 2004, pp. 1739-1745
P. De Taxi du Poet, S. Miyamoto, T. Murakami, J. Kiura, I.. Karube, “Direct electron transfer with glucose oxidase immobilized in an electropolymerized poly(N-methylpyrrole) film on a gold electrode”, Analytica Chimica Acta, vol. 235, Aug. 1990, pp. 255 – 264
D.W. Kolpin, E.T. Furlong, M.T. Meyer, E.M. Thurman, S.D. Zaugg, L.B. Barber, H.T. Buxton, “Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance”, Environmental Science and Technology, vol. 36, Mar. 2002, pp. 1202-1211
A. Stafiej, K. Pyrzynska, F. Regan, “Determination of anti‐inflammatory drugs and estrogens in water by HPLC with UV detection”, Journal of Separation Science, vol. 30, Apr. 2007, pp. 985-991
P. Pander, R. Motyka, P. Zassowski, M. Lapkowski, A. Swist, P. Data, “Electrochromic Properties of Novel Selenophene and Tellurophene Derivatives Based on Carbazole and Triphenylamine Core”, The Journal of Physical Chemistry C, vol. 121, May 2017, pp. 11027−11036
F. Rusmini, Z. Zhong, J. Feijen, “Protein immobilization strategies for protein biochips”, Biomacromolecules, vol. 8, Jun. 2007, pp. 1775–1789
I.W. Berezin, N.L. Kliaczko, A.W. Lewaszow, K. Martinek, W.W. Możajew, I. Chmielnicki, „Immobilizowane enzymy”, Biotechnologia T. 7, Uniwersytet im. Adama Mickiewicza, Poznań, 1992
A. Sassolas, L.J. Blum, B.D. Leca-Bouvier, “Immobilization strategies to develop enzymatic biosensors”, Biotechnology Advances, vol. 30, May-Jun. 2012, pp. 489–511
J. Cabaj, J. Sołoducho, A. Jędrychowska, D. Zając, “Biosensing invertase-based Langmuir–Schaefer films: Preparation and characteristic”, Sensors and Actuators B, vol. 166–167, May 2012, pp. 75–82
T.C. Gokoglan, S. Soylemez, M. Kesik, S. Toksabay, L. Toppare, “Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection”, Food Chemistry, vol. 172, Apr. 2015, pp. 219-224
T. Catal, H. Liu, H. Bermek, “Selenium Induces Manganese-dependent Peroxidase Production by the White-Rot Fungus Bjerkandera adusta (Willdenow) P. Karsten”, Biological Trace Element Research, vol. 123, Jun. 2008, pp. 211–217
E. Desimoni, B. Brunetti, “Presenting Analytical Performancesof Electrochemical Sensors. Some Suggestions”, Electroanalysis, vol. 25, Jun. 2013, pp. 1645-1651
J. Wei, J. He, C. Chen, X. Wang, “A catechin-modified carbon paste electrode forelectrocatalytic determination ofneurotransmitters”, Analytical Methods, vol. 7, May 2015, pp. 5641-5648
S. Liu, F. Shi, X. Zhao, L. Chen, X. Su, “3-Aminophenyl boronic acid-functionalized CuInS2 quantum dots as a near-infrared fluorescence probe for the determination of dopamine” Biosensors and Bioelectronics, vol. 47, Sep. 2013, pp. 379–384
S. Weng, D. Liang, H. Qiu, Z. Liu, Z. Lin, Z. Zheng, A. Liu, W. Chen, X. Lin, “A unique turn-off fluorescent strategy for sensing dopamine based on formed polydopamine (pDA) using graphene quantum dots (GQDs) as fluorescent probe”, Sensors and Actuators B, vol. 221, Dec. 2015, pp. 7–14
A. Mohammadi, A. Bayandori Moghaddam, S. Hosseini, M. Kazemzade, R. Dinarvandf, “A norepinephrine biosensor based on a glassy carbon electrode modified withcarbon nanotubes”, Analytical Methods, vol. 3, Aug. 2011, pp. 2406-2411
J.T. Moraes, C.A. Salamanca-Neto, L. Švorc, J.G. Schirmann, A. M Barbosa-Dekker, R. Dekker, E. R Sartori, “Laccase from Botryosphaeria rhodina MAMB-05 as a biological component in electrochemical biosensing devices”, Analytical Methods, vol. 11, Feb. 2019, pp. 717–720
Downloads
Published
Issue
Section
License
Copyright (c) 2020 International Journal of Electronics and Telecommunications
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on https://creativecommons.org/licenses/by/4.0/.
2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author/s. The undersigned also warrants that the manuscript (or its essential substance) has not been published other than as an abstract or doctorate thesis and has not been submitted for consideration elsewhere, for print, electronic or digital publication.
3. User Rights
Under the Creative Commons Attribution license, the author(s) and users are free to share (copy, distribute and transmit the contribution) under the following conditions: 1. they must attribute the contribution in the manner specified by the author or licensor, 2. they may alter, transform, or build upon this work, 3. they may use this contribution for commercial purposes.
4. Rights of Authors
Authors retain the following rights:
- copyright, and other proprietary rights relating to the article, such as patent rights,
- the right to use the substance of the article in own future works, including lectures and books,
- the right to reproduce the article for own purposes, provided the copies are not offered for sale,
- the right to self-archive the article
- the right to supervision over the integrity of the content of the work and its fair use.
5. Co-Authorship
If the article was prepared jointly with other authors, the signatory of this form warrants that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.
6. Termination
This agreement can be terminated by the author or the Journal Owner upon two months’ notice where the other party has materially breached this agreement and failed to remedy such breach within a month of being given the terminating party’s notice requesting such breach to be remedied. No breach or violation of this agreement will cause this agreement or any license granted in it to terminate automatically or affect the definition of the Journal Owner. The author and the Journal Owner may agree to terminate this agreement at any time. This agreement or any license granted in it cannot be terminated otherwise than in accordance with this section 6. This License shall remain in effect throughout the term of copyright in the Work and may not be revoked without the express written consent of both parties.
7. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by the Journal Owner or its sublicensee.
8. Miscellaneous
The Journal Owner will publish the article (or have it published) in the Journal if the article’s editorial process is successfully completed and the Journal Owner or its sublicensee has become obligated to have the article published. Where such obligation depends on the payment of a fee, it shall not be deemed to exist until such time as that fee is paid. The Journal Owner may conform the article to a style of punctuation, spelling, capitalization and usage that it deems appropriate. The Journal Owner will be allowed to sublicense the rights that are licensed to it under this agreement. This agreement will be governed by the laws of Poland.
By signing this License, Author(s) warrant(s) that they have the full power to enter into this agreement. This License shall remain in effect throughout the term of copyright in the Work and may not be revoked without the express written consent of both parties.
